Publication Highlights

J.A. De Lora†, J.L. Velasquez, N.J. Carroll, J.P. Freyer, A.P. Shreve†, "Centrifugal generation of droplet-based 3D cell cultures," SLAS Technology, 25 (2020) 436-445, doi: 10.1177/2472630320915837. (†Co-corresponding authors)

This paper demonstrates the use of centrifugal generation to produce large numbers of cell-containing hydrogel droplets to enable 3D cell culture studies.

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V. Subramanian, N.A. Zurek, D.G. Evans, A.P. Shreve*, "Predictive modeling of broad wavelength light-harvesting performance in assemblies of multiple chromophores," J. Photochem. Photobiol. A: Chemistry,361, 2018, 105-114 (doi: 10.1016/j.jphotochem.2018.08.007)

This paper demonstrates computationally efficient design of random assemblies of multiple types of chromophores for panchromatic light-harvesting, developed using an approximate modeling approach validated against both experimental studies and numerical computational methods. Based on these results, by using tabulated pairwise Förster radius values and spectral data, researchers can easily design self-assembled light-harvesting systems with desired spectral performance.

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D.M. Kalb, F.A. Fencl, T.A. Woods, A. Swanson, G.C. Maestas, J.J. Juárez, B.S. Edwards, A.P. Shreve*, S.W. Graves*, "Line-focused optical excitation of parallel acoustic focused sample streams for high volumetric and analytical rate flow cytometry," Analytical Chemistry,89, 2017, 9967-9975 (doi: 10.1021/acs.analchem.7b02319)

This paper reports use of acoustic standing wave focusing to produce multiple parallel streams of particles in a laser-interrogated flow chamber, allowing particle analysis rates of ≈100,000 particles per second and volumetric flow rates of 10 mL/min. This performance greatly exceeds both the particle analysis rates and volumetric flow rates of existing flow cytometers, and does so using an instrument design that is compact and easy to implement.

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N. Fernandez Oropeza, N.A. Zurek, M. Galvan-De La Cruz, A. Fabry-Wood, J.M. Fetzer, S.W. Graves*, A.P. Shreve*, "Multiplexed lipid bilayers on silica microspheres for analytical screening applications," Analytical Chemistry,89, 2017, 6440-6447 (doi: 10.1021/acs.analchem.7b00296)

This work presents proof-of-principle results for how flow-cytometry-based assays involving membrane-associated components can be implemented in a multiplexed format. The approach relies on building multiplexed sets of substrate-supported lipid membranes of different compositions on silica microspheres, enabling multiple assays to be performed in a single container.

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P.G. Adams, A.M. Collins, T. Sahin, V. Subramanian, V.S. Urban, P. Vairaprakash, Y. Tian, D.G. Evans, A.P. Shreve*, G.A. Montaño, "Diblock copolymer micelles and supported films with noncovalently incorporated chromophores: A modular platform for efficient energy transfer," Nano Letters,15, 2015, 2422-2428 (doi: 10.1021/nl504814x)

This paper demonstrates synthetic light-harvesting assemblies where an amphiphilic diblock copolymer serves as the organizing material for donor and acceptor energy-transfer pairs. Performance of the assemblies can be tuned through adjustment of the mesoscale structures generated by the block copolymer, enabling development of chromophore-containing polymer-based mesoscale assemblies for light-harvesting and photonic applications.

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